Measuring mitochondrial function and glycolysis using the Seahorse XF analyzers

Ponente: Alfredo Caro-Maldonado

Seahorse XF Consumables Product Specialist Cell Analysis, Agilent Technologies

Host: -

Fecha: 05/09/2019

Hora: 12:00

Salón de actos del Centro de Investigación del Cáncer

Mitochondrial function and glycolysis play critical roles in a variety of vital cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. Seahorse Bioscience has developed a technology that enables the measurement of various metabolic parameters and functions using live cells, in real-time, in a microplate. Seahorse Analyzers profile cellular metabolic functions, using label-free, solid-state disposable optical sensors. The Seahorse Analyzers simultaneously measure mitochondrial respiration (oxidative phosphorylation; OXPHOS) via the oxygen consumption rate (OCR), and glycolysis via the extracellular acidification rate (ECAR). Also the Seahorse Analyzer is able to measure and quantifies the rate of ATP (adenosine triphosphate) production from glycolysis and mitochondria simultaneously using label-free technology in live cells, in real time. Real-time kinetic quantification delivers a dynamic picture of cellular bioenergetics, providing researchers with unique insight into cellular phenotype and function. Integrated drug injection ports allow for up to 4 reagent additions (e.g. drug or substrate) that can be programmed for automated delivery into the independent cell culture wells. Assay kits and reagents provide standard methods for quantifying mitochondrial respiration, glycolytic activity, endogenous and exogenous fatty acid oxidation, substrate oxidation, ATP rate production, and metabolic phenotype. Seahorse XF technology has been applied to multiple research areas, including cancer, obesity, diabetes, metabolic disorders, immunology, cardiovascular function, neurodegeneration, virology, and aging.