Servicio de Citogenética Molecular


Molecular Cytogenetics Unit (MCU) is a facility devoted to the karyotypic analysis, fluorescence “in situ” hybridization, comparative genomic hybridization, microarrays and nextgeneration sequencing of cancer patients. Most than 100 hospitals in Spain, and occasionally others from the EU,
have used the MCU services.  The Unit collaborates with the most relevant groups in the treatment of the hematological malignancies such as Pethema, GEL-TAMO, GEM or GETH providing technical support and the characterization of the genetic abnormalities in the patients included in clinical
trials. In addition, the MCU is involved in several international projects related to expression microarrays (MILE), genomic microarrays (EuGESMA), and next generation sequencing


• Bone Marrow Cytogenetics: leukemia, lymphoma and myeloma
• Peripheral blood cytogenetics: leukemia, lymphoma and myeloma
• Lymph node and spleen cytogenetics: lymphoma
• Solid tumour cytogenetics
• Centromeric ‘in situ’ hybridization: FISH performerd with centromeric probes to analyse numerical
• Painting ‘in situ’ hybridization : FISH performed with libraries of DNA to analyse structural abnormalities
• Loci specific ‘in situ’ hybridizations: FISH performed with probes to analyse either losses or gains of genetic material or fusion genes.
• Comparative Genomic Hybridization: Test to analyse global gains and losses of genetic material in tumour cells.
• MultiFISH
• Genomic microarrays
• Farmacogenetic microarrays
• Next generation sequencing


• Full authomatised system for karyotyping and FISH
(Cytovision) with 3 analysis stations
• Full authomatised system for karyotyping and FISH
(Metasystems) including a karyotype finder with 3
analysis stations
• Microbeads-based system for cellular isolation (Miltenyi)
• Microscopes of light and fluorescence
• Pirosequencer


Molecular Cytogenetics Service: Jesús María Hernández Rivas, Norma C. Gutiérrez, Juan Luis García Hernández, Eva Lumbreras González, Cristina Robledo Montero, Rocío Benito Sánchez, María del Pozo Hernández, Ana Belén Díaz Martín, Micaela Fonseca García, Sara González Briones, Mª Ángeles Hernández García, María Almudena Martín Martín, Mª Ángeles Ramos Rodríguez, Irene Rodríguez Iglesias, Ana María Simón Muñoz, Isabel Mª Isidro Hernández, Vanesa Gutiérrez Moreta, Mª Teresa Prieto Martín


Scientific Coordinator:

Jesús María Hernández Rivas (Molecular Cytogenetics Service)
Phone: (+34) 923 291 100 Ext. 55764
E-mail: jmhr@usal.es
Marcos González Díaz (Molecular Biology Service)
Phone: (+34) 923 291 100 Ext. 55629
E-mail: margondi@usal.es

Location and contact:

Unidad de Diagnóstico Molecular y Celular
Centro de Investigación del Cáncer (CSIC-USAL)
Campus Universitario Miguel de Unamuno s/n E- 37007 Salamanca (ESPAÑA)
Jesús María Hernández Rivas (Molecular Cytogenetics Service)
Phone: (+34) 923 291 100 Ext. 55764
E-mail: jmhr@usal.es